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- Title
Sinensetin ameliorates high glucose‐induced diabetic nephropathy via enhancing autophagy in vitro and in vivo.
- Authors
Kong, Zili; Lv, Wenshan; Wang, Yunyang; Huang, Yajing; Che, Kui; Nan, Huiqi; Xin, Yu; Wang, Jiaxuan; Chen, Jintao; Wang, Yangang; Chi, Jingwei
- Abstract
Diabetic nephropathy (DN) affects around 40% of people with diabetes, the final outcome of which is end‐stage renal disease. The deficiency of autophagy and excessive oxidative stress have been found to participate in the pathogenesis of DN. Sinensetin (SIN) has been proven to have strong antioxidant capability. However, the effect of SIN on DN has not been studied. We examined the effect of SIN on cell viability and autophagy in the podocyte cell line, MPC5 cells, treated with high glucose (HG). For in vivo studies, DN mice models were established by intraperitoneal injected with streptozotocin (40 mg/kg) for 5 consecutive days and fed with a 60% high‐fat diet, and SIN was given (10, 20, and 40 mg/kg) for 8 weeks via intraperitoneal injection. The results showed that SIN could protect MPC5 cells against HG‐induced damage and significantly improve the renal function of DN mice. Moreover, SIN remarkably restored the autophagy activity of MPC5 cells which was inhibited under HG conditions. Consistent with this, SIN efficiently improved autophagy in the kidney tissue of DN mice. In brief, our findings demonstrated the protective effect of SIN on DN via restoring the autophagic function, which might provide a basis for drug development. Highlights: Sinensetin (SIN) could protect podocytes from high glucose (HG) injury.SIN could restore the autophagic function injured by HG.SIN could protect the mitochondria of podocytes, kidney damage, and damaged autophagy in the experimental diabetic nephropathy mice model.
- Subjects
DIABETIC nephropathies; AUTOPHAGY; CHRONIC kidney failure; PEOPLE with diabetes; HIGH-fat diet; KIDNEY physiology
- Publication
Journal of Biochemical & Molecular Toxicology, 2023, Vol 37, Issue 10, p1
- ISSN
1095-6670
- Publication type
Article
- DOI
10.1002/jbt.23445