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- Title
Disturbed enamel biomineralization in col1-caPPR mouse incisor.
- Authors
Zhen-Jiang Cheng; Xiu-Mei Wang; Jun Ge; Di Chen; Fu-Zhai Cui; Cheng, Zhen-Jiang; Wang, Xiu-Mei; Ge, Jun; Chen, Di; Cui, Fu-Zhai
- Abstract
During the mineralization process of enamel, gene expression controls the activities of ameloblasts, the secretion and assembly of an extracellular protein matrix, affecting the final structure and functions. In this study, the enamel in the maxillary and mandibular incisors of wild-type and transgenic (col1-caPPR) mice, in which a constitutively active PTH/PTHrP receptor (PPR) was targeted to osteoblastic cells, was observed by scanning electron microscopy (SEM), Fourier transform infrared microscopy (FTIRM), and nanoindentation. The SEM studies showed that several different patterns of aberrations in crystal arrangement, disturbed prism organization without decussation, as well as abnormal enamel distribution were encountered in transgenic enamel. FTIRM analysis revealed poorer crystallinity/maturity after mutation. Nanoindentation measurement disclosed that transgenic enamel had 24.6% lower hardness and 12.3% lower elastic modulus. We attributed the inferior properties to the loosely packing crystals and abnormal prism organization. Furthermore, the col1-caPPR mouse model was substantiated to be useful to study how genes modulate the biomineralization process.
- Subjects
BIOMINERALIZATION; MINERALS in the body; GENE expression; EXTRACELLULAR matrix; ELECTRON microscopy; CELL receptors; ANIMAL experimentation; BONE growth; COMPARATIVE studies; DENTAL enamel; INCISORS; INFRARED spectroscopy; RESEARCH methodology; MEDICAL cooperation; MICE; RESEARCH; SCANNING electron microscopy; EVALUATION research; CELL physiology
- Publication
Calcified Tissue International, 2009, Vol 84, Issue 6, p494
- ISSN
0171-967X
- Publication type
journal article
- DOI
10.1007/s00223-009-9243-9