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- Title
An efficient protocol for plant regeneration from protoplasts of the moss Atrichum undulatum P. Beauv in vitro.
- Authors
Lin-Hui Li; Xian-Ping Wang; Wan-Ru Hou; Xiang-Lin Liu; Yi-Kun He
- Abstract
Abstract A system for plant regeneration from protoplasts of the moss, Atrichum undulatum (Hedw.) P. Beauv. in vitro, is first reported. Viable protoplasts were isolated at about 9× 105 protoplasts g-1 fresh weight from 10 to 18days protonemata. For regeneration of protoplasts, viable protoplasts were cultured in liquidsolid medium containing surface liquid medium MS (0.4M mannitol) and subnatant solid medium Benecke (0.3M mannitol) at 20C under a 16-h photoperiod white light after 12h preculture in darkness at 20C. The great majority of protoplasts follow a regenerative sequence: formation of asymmetric cells in 23days; division of the asymmetric cells to 23 cells in 45days, and further develop to produce a new chloronemal filament in 15days. Juvenile gametophyte can be visible in 20days. The plating ratio of cell cluster regenerated from protoplasts reaches up to 45%. Transient expression experiments indicate the electroporation uptake of DNA is possible.
- Subjects
PROTOPLASTS; CYTOLOGICAL techniques; PROTONEMATA; BIOELECTROCHEMISTRY
- Publication
Plant Cell, Tissue & Organ Culture, 2005, Vol 82, Issue 3, p281
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-005-1370-z