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- Title
A novel preparation method for a proteoglycan in a matrix with collagen from salmon (Oncorhynchus keta) nasal cartilage and its affinity to L-selectin.
- Authors
Yusuke Okamoto; Kyohei Higashi; Toshihiko Toida
- Abstract
An intact proteoglycan was extracted from salmon (Oncorhynchus keta) nasal cartilage containing type II collagen and prepared using a novel extraction procedure in water containing a sugar fatty acid ester as an edible detergent. This isolation step suppressed the degradation of the proteoglycan and simultaneously afforded a proteoglycan-type II collagen matrix. The extracted proteoglycan was purified, and its properties were compared with those prepared via different extraction procedures using gel permeation chromatography and polyacrylamide gel electrophoresis. Furthermore, the interaction between the purified proteoglycan and human L-selectin was analyzed using a bio-layer interferometry biosensor assay; the proteoglycan demonstrated strong binding to L-selectin.
- Subjects
CHUM salmon; PROTEOGLYCANS; FATTY acid esters; GEL permeation chromatography; POLYACRYLAMIDE gel electrophoresis
- Publication
Japanese Journal of Food Chemistry & Safety, 2021, Vol 28, Issue 1, p9
- ISSN
1341-2094
- Publication type
Article