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- Title
MDA-MB-231 and 8701BC breast cancer lines promote the migration and invasiveness of ECV304 cells on 2D and 3D type-I collagen matrix.
- Authors
Saladino, Silvia; Salamone, Monica; Ghersi, Giulio
- Abstract
Tumor angiogenesis is a multiphasic process, having the extracellular matrix remodeling as critical step. Different classes of proteolytic enzymes in matrix digestion/remodeling are involved. The role of lytic enzymes and their activation mode have not been completely elucidated. Herein, the crosstalk between endothelia and tumor cells, by realization of bi- and three-dimensional endothelial and breast cancer cells co-cultures, were studied in vitro. Particularly, the effects of two tumor conditioned media (TCM) were assessed about endothelial proliferation, migration, and invasiveness. An increase in expression of pro-MMP9 was detected when endothelial cells were cultured in the presence of both TCM; such as an up-regulation of MMP1 and MMP14 and a down-regulation of MMP7. Moreover the increased MMP2 gene expression from one of them and the stimulation MMP3 synthesis from the other one were observed; an increases of β3-integrin, VEGFA, and DPP4 molecules were detected when endothelia cells are cultured with both TCM. The selection/characterization of elements present in conditioned media from breast cancer cells differently affect endothelial cells, make them potential effectors useful in breast cancer treatment.
- Subjects
CANCER cell culture; TUMOR blood vessels; EXTRACELLULAR matrix; ENDOTHELIAL cells; CELL migration; CANCER invasiveness; MATRIX metalloproteinases; BREAST cancer research
- Publication
Cell Biology International, 2017, Vol 41, Issue 9, p1030
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10817