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- Title
Separation and Preliminary Identification of Intestinal Probiotics of Laying Hens.
- Authors
Guo Shijin; Zhang Zhimei; Fu Shijun; Mo Ling; Zhang Ying; Shen Zhiqiang; Huang Baohua
- Abstract
[Objective] The paper was to isolate and identify probiotics in the intestine of laying hens. [Method] The intestinal probiotics in laying hens at peak period were isolated using conventional separation methods; the physical and chemical properties of target strains and in vitro antibacterial effects were measured. Moreover, the safety test of chicks was conducted. [Result] Four strains of lactobacillus were isolated from the jejunum of laying hens, including Lactobacillus plantarum, Bacillus acidophilus, L. delbrueckii subsp. Delbrueckii and L. delbrueckii subsp. Lactis, and their inhibition zone diameters were 18.30, 16.07, 11.45, 17.26 mm, respectively. One strain of Lactobacillus, L. brevis, was isolated from the cecum, with the inhibition zone diameter of 10.26 mm. Three strains of bacillus were isolated from the cecum, including Bacillus subtilis, B. cereus and B. licheniformis, and their inhibition zone diameters were 9.25, 8.46 and 8.37 mm, respectively. Daily drinking 2 billion units of viable bacteria was the safe dosage for chicks. [Conclusion] Eight strains of probiotics had certain inhibitory effect on Escherichia coli O2, and had no toxic side effects to chicks. Lactobacillus had strong antibacterial effect on E. coli O2, while the antibacterial effect of bacillus was relatively weak.
- Subjects
POULTRY physiology; GUT microbiome; PROBIOTICS; LACTOBACILLUS plantarum; SEPARATION (Technology)
- Publication
Animal Husbandry & Feed Science, 2018, Vol 10, Issue 1, p72
- ISSN
1943-9911
- Publication type
Article
- DOI
10.19578/j.cnki.ahfs.2018.01.016