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- Title
An Rtf2 Domain-Containing Protein Influences Pre-mRNA Splicing and Is Essential for Embryonic Development in Arabidopsis thaliana.
- Authors
Taku Sasaki; Tatsuo Kanno; Shih-Chieh Liang; Pao-Yang Chen; Wen-Wei Liao; Wen-Dar Lin; Matzke, Antonius J. M.; Matzke, Marjori
- Abstract
Alternative splicing is prevalent in plants, but little is known about its regulation in the context of developmental and signaling pathways. We describe here a newfactor that influences pre-messengerRNA (mRNA) splicing and is essential for embryonic development in Arabidopsis thaliana. This factor was retrieved in a genetic screen that identified mutants impaired in expression of an alternatively spliced GFP reporter gene. In addition to the known spliceosomal component PRP8, the screen recovered Arabidopsis RTF2 (AtRTF2), a previously uncharacterized, evolutionarily conserved protein containing a replication termination factor 2 (Rtf2) domain. A homozygous null mutation in AtRTF2 is embryo lethal, indicating that AtRTF2 is an essential protein. Quantitative RT-PCR demonstrated that impaired expression of GFP in atrtf2 and prp8 mutants is due to inefficient splicing of the GFP pre-mRNA. A genome-wide analysis using RNA sequencing indicated that 13-6% of total introns are retained to a significant degree in atrtf2 mutants. Considering these results and previous suggestions that Rtf2 represents an ubiquitin-related domain, we discuss the possible role of AtRTF2 in ubiquitin-based regulation of pre-mRNA splicing.
- Subjects
ALTERNATIVE RNA splicing; EMBRYOLOGY; ARABIDOPSIS thaliana; ZINC-finger proteins; PLANTS
- Publication
Genetics, 2015, Vol 200, Issue 2, pS23
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1534/genetics.115.176438