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- Title
Pteridine glycosyltransferase from Chlorobium tepidum: crystallization and X-ray analysis.
- Authors
Killivalavan, Asaithambi; Park, Young Shik; Lee, Kon Ho
- Abstract
The pteridine glycosyltransferase (PGT) found in Chlorobium tepidum ( CtPGT) catalyzes the conversion of l- threo-tetrahydrobiopterin to 1- O-( l- threo-biopterin-2′-yl)-β- N-acetylglucosamine using UDP- N-acetylglucosamine. The gene for CtPGT was cloned, and selenomethionine-derivatized protein was overexpressed and purified using various chromatographic techniques. The protein was crystallized by the hanging-drop vapour-diffusion method using 0.24 M triammonium citrate pH 7.0, 14%( w/ v) PEG 3350 as a reservoir solution. Multiple-wavelength anomalous diffraction data were collected to 2.15 Å resolution from a single CtPGT crystal. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 189.61, b = 79.98, c = 105.92 Å, β = 120.5°.
- Subjects
PTERIDINES; GLYCOSYLTRANSFERASES; CHLOROBIUM tepidum
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2017, Vol 73, Issue 11, p629
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X17015515