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- Title
Application of Median Nerve Electrical Stimulation to Restore Neuronal Function and Promote Myelin Regeneration after Stroke in Rats.
- Authors
Rui Li; Jingyi Lu; Chengcai Zhang; Pengyue Zhang; Kai Ling Chin
- Abstract
Introduction: Upper limb motor dysfunction is one of the common complications of stroke patients. This symptom is associated with disruption of normal neuronal function. Restoration of the upper limb motor dysfunction following stroke remains a major challenge for rehabilitation medicine. Clinically, we had demonstrated that median nerve electrical stimulation (MNES) could significantly improve the upper limb motor function in stroke patients, but the underlying mechanism is still unclear. Myelin regeneration plays an important role in the restoration of nerve function in stroke. In this study, we aimed to investigate the effects of MNES on myelin regeneration in a rat stroke model. Methods: Adult male Sprague-Dawley rats (n=24) were divided into three groups, i.e., sham group, left middle cerebral artery occlusion stroke model (MCAO group), and stroke model receiving MNES treatment (MNES group). The MNES group received MNES intervention (once per day for 7 times) on the injured side forelimb at 3 days after MCAO. The neural functional recovery was evaluated by neurological severity score (NSS), Rotarod (RR), and foot fault test (FFT). The expression of myelin basic protein (MBP) was detected by Western blotting. The myelin thickness and number of medullated fibers in the penumbra area were observed by transmission electron microscopy (TEM). Results: The MNES group had significantly improved the performance of NSS, RR, and FFT scores compared to the MCAO group (p<0.01). Also, the expression level of MBP protein in the MNES group was increased (p<0.05). Under TEM, compared to the MCAO group, the MNES group had increased myelin thickness (p<0.01) and number of medullated fibers (p<0.05), the shape of nerve fibers was regular, and the lamellar structure was dense. Conclusion: The MNES treatment could help in restoring neuronal function by promoting the expression of MBP, myelin regeneration, and improving the microstructure of myelin after stroke.
- Subjects
MEDIAN nerve; NEURAL stimulation; STROKE; MYELIN; MYELIN basic protein
- Publication
Malaysian Journal of Medicine & Health Sciences, 2024, Vol 20, p46
- ISSN
1675-8544
- Publication type
Article