Protozoan parasites are among the most common pathogens. Leishmania affects large populations of the world especially in the third world countries including Pakistan. We attempted to assess synthesized 1,3-disubstituted ureas for leishmanicidal activity. Compounds, N-(2,6-dimethylphenyl)-N’-(2-nitrophenyl) urea and N-butyl-N’-(4-nitrophenyl)urea showed pronounced inhibition with IC50 ± S.D (μg/mL) of 29.16 ± 1.04 and 40.91 ± 0.12, respectively.