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- Title
Production of a proteinaceous phytotoxin by <em>Ascochyta rabiei</em> grown in expressed chickpea sap.
- Authors
Chen, Y.-M.; Strange, R. N.
- Abstract
Production of the solanapyrone toxins by Ascochyta rabiei is nutrient dependent. When grown on a medium consisting entirely of expressed sap from the aerial parts of young chickpea plants (PSM), only low concentrations of the solanapyrones were produced (< 24 μM). However, toxicity of 4-day-old culture filtrates to isolated cells of chickpea leaflets was comparable with that obtained from 12-day-old culture filtrates on Czapek Dox nutrients supplemented with chickpea seed extract or cations-media that are conducive to solanapyrone production. The additional toxic component which peaked at 4 days in culture was heat labile, losing about 50% of its activity on boiling for 10 min. Affinity for solid-phase extraction media, precipitation with ammonium sulphate and acetone, ionization properties and UV absorption characteristics suggested that the toxin was a polypeptide. The toxin was purified by solid-phase extraction, acetone precipitation and high performance liquid chromatography (HPLC) on a C2 column. Hydrolysis of the purified toxin yielded 14 amino acids, and calculation of the numbers of residues of each amino acid suggested a molecular mass of 7,551 Da. A band corresponding to this molecular mass was present on SDS-PAGE gels. However, both the native peptide and its hydrolysate contained a compound that reacted with p-anisaldehyde suggesting the possibility of a glycosidic moiety.
- Subjects
PRIONS; PHYTOTOXINS; PHYTOTOXICITY; CHICKPEA; ASCOCHYTA rabiei; AMINO acids
- Publication
Plant Pathology, 1994, Vol 43, Issue 2, p321
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.1994.tb02691.x