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- Title
Germ cell-specific expression of Cre recombinase using the VASA promoter in the pig.
- Authors
Song, Yuning; Lai, Liangxue; Li, Li; Huang, Yongye; Wang, Anfeng; Tang, Xiaochun; Pang, Daxin; Li, Zhanjun; Ouyang, Hongsheng
- Abstract
The Cre- loxP system is a powerful tool for genetic analysis of distinct cell lineages and tissue-specific gene knockout in animal models. VASA is specifically expressed in reproductive tissues, and is known to play important roles in spermatogenesis and germ-cell growth. In this study, Cre recombinase transgenic pigs under the control of the VASA promoter were generated by somatic cell nuclear transfer. Germ cell-specific expression of Cre recombinase in VASA-Cre transgenic pigs was shown by western blotting and immunohistochemistry. VASA-Cre transgenic pigs will be a useful tool for germ cell-specific gene knockout and a disease model for disorders of the reproductive system.
- Subjects
GERM cells; GENE expression; RECOMBINASES; PROMOTERS (Genetics); TRANSPLANTATION of cell nuclei; IMMUNOHISTOCHEMISTRY; LABORATORY swine
- Publication
FEBS Open Bio, 2016, Vol 6, p50
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1002/2211-5463.12005