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- Title
Kilohertz Macromolecular Crystallography Using an EIGER Detector at Low X-ray Fluxes.
- Authors
Khakurel, Krishna P.; Espinoza, Shirly; Savko, Martin; Polovinkin, Vitaly; Dohnalek, Jan; Shepard, William; Angelova, Angelina; Hajdu, Janos; Andreasson, Jakob; Angelov, Borislav
- Abstract
Time-resolved in-house macromolecular crystallography is primarily limited by the capabilities of the in-house X-ray sources. These sources can only provide a time-averaged structure of the macromolecules. A significant effort has been made in the development of in-house laser-driven ultrafast X-ray sources, with one of the goals as realizing the visualization of the structural dynamics of macromolecules at a very short timescale within the laboratory-scale infrastructure. Most of such in-house ultrafast X-ray sources are operated at high repetition rates and usually deliver very low flux. Therefore, the necessity of a detector that can operate at the repetition rate of the laser and perform extremely well under low flux conditions is essential. Here, we present experimental results demonstrating the usability of the hybrid-pixel detectors, such as Eiger X 1M, and provide experimental proof that they can be successfully operated to collect macromolecular crystallographic data up to a detector frame rate of 3 kHz from synchrotron sources. Our results also show that the data reduction and structural analysis are successful at such high frame rates and fluxes as low as 108 photons/s, which is comparable to the values expected from a typical laser-driven X-ray source.
- Subjects
X-rays; DETECTORS; CRYSTALLOGRAPHY; STRUCTURAL dynamics; FLUX (Energy)
- Publication
Crystals (2073-4352), 2020, Vol 10, Issue 12, p1146
- ISSN
2073-4352
- Publication type
Article
- DOI
10.3390/cryst10121146