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- Title
Draft genome sequence of <italic>Penicillium chrysogenum</italic> strain HKF2, a fungus with potential for production of prebiotic synthesizing enzymes.
- Authors
Gujar, Vaibhav V.; Fuke, Priya; Khardenavis, Anshuman A.; Purohit, Hemant J.
- Abstract
In this study, we have characterized a novel set of extracellular enzymes produced by <italic>Penicillium chrysogenum</italic> strain HKF2. A draft genome data of 31.5 Mbp was generated and annotation suggested a total of 11,243 protein-coding genes out of which 609 were CAZymes, majority of which were found to have homology with <italic>Penicillium rubens, Penicillium chrysogenum</italic> followed by <italic>Penicillium expansum</italic> and <italic>Penicillium roqueforti</italic>. The prominent CAZyme genes identified in the draft genome encoded for enzymes involved in the production of prebiotics such as inulo-oligosaccharides and fructo-oligosaccharides. Corresponding enzyme assay indicated that the isolate possessed the potential to produce 11.8 and 3.8 U/mL of β-fructofuranosidase and inulinase, respectively. This study highlights the significance of Effluent Treatment Plants as novel and under-explored niche for isolation of fungi having the potential for production of prebiotics synthesizing enzymes.
- Subjects
NUCLEOTIDE sequencing; PENICILLIUM chrysogenum; FUNGI; POLYSACCHARIDES; MICROORGANISMS
- Publication
3 Biotech, 2018, Vol 8, Issue 2, p0
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-018-1132-3