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- Title
Development of microsatellite DNA markers of grass carp (Ctenopharyngodon idella) and their cross-species application in black carp (Mylopharyngodon piceus).
- Authors
Shanshan Guo; Guiwei Zou; Guanpin Yang
- Abstract
Thirty-four microsatellite DNA loci were developed for grass carp (Ctenopharyngodon idella) and used to determine the diversity of 42 individuals of grass carp and 16 individuals of black carp collected from Jingzhou fragment (the middle reach) of Yangtze River. All the 34 loci were polymorphic in grass carp. The number of alleles found in grass carp at these loci ranged from 2 to 24. The observed and expected heterozygosities varied from 0.238 to 1.000 and from 0.162 to 0.945, respectively. Thirty of the 34 loci cross-amplified the DNA of black carp (Mylopharyngodon piceus), and 21 of them revealed polymorphism (more than one allele each locus).
- Subjects
CHINA; CTENOPHARYNGODON idella; BLACK carp; GENETIC polymorphisms; GENETIC markers
- Publication
Conservation Genetics, 2009, Vol 10, Issue 5, p1515
- ISSN
1566-0621
- Publication type
Article
- DOI
10.1007/s10592-008-9777-9