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- Title
Uncultured D esulfobacteraceae and Crenarchaeotal group C3 incorporate <sup>13</sup> C-acetate in coastal marine sediment.
- Authors
Na, Hyunsoo; Lever, Mark Alexander; Kjeldsen, Kasper Urup; Schulz, Frederik; Jørgensen, Bo Barker
- Abstract
Stable isotope probing ( SIP) of deoxyribonucleic acid ( DNA) was used to identify microbes incorporating 13 C-labeled acetate in sulfate-reducing sediment from Aarhus Bay, Denmark. Sediment was incubated in medium containing 10 mM sulfate and different 13 C-acetate (10, 1, 0.1 mM) concentrations. The resultant changes in microbial community composition were monitored in total and SIP-fractionated DNA during long-term incubations. Chemical analyses demonstrated metabolic activity in all sediment slurries, with sulfate-reducing activity largely determined by initial acetate concentrations. Sequencing of 16 S r RNA gene PCR amplicons showed that the incubations shifted the bacterial but not the archaeal community composition. After 3 months of incubation, only sediment slurries incubated with 10 mM 13C-acetate showed detectable 13 C- DNA labeling. Based on 16 S r RNA and dsr B gene PCR amplicon sequencing, the 13 C-labeled DNA pool was dominated by a single type of sulfate reducer representing a novel genus in the family D esulfobacteraceae. In addition, members of the uncultivated Crenarchaeotal group C3 were enriched in the 13 C-labeled DNA. Our results were reproducible across biological replicate experiments and provide new information about the identities of uncultured acetate-consuming bacteria and archaea in marine sediments.
- Subjects
MARINE bacteria; BACTERIAL cultures; MARINE sediment microbiology; BACTERIAL DNA; SULFATE-reducing bacteria
- Publication
Environmental Microbiology Reports, 2015, Vol 7, Issue 4, p614
- ISSN
1758-2229
- Publication type
Article
- DOI
10.1111/1758-2229.12296