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- Title
Fracture healing on non-union fracture model promoted by non-thermal atmospheric-pressure plasma.
- Authors
Saito, Kosuke; Toyoda, Hiromitsu; Okada, Mitsuhiro; Oh, Jun-Seok; Nakazawa, Katsumasa; Ban, Yoshitaka; Orita, Kumi; Shimatani, Akiyoshi; Yao, Hana; Shirafuji, Tatsuru; Nakamura, Hiroaki
- Abstract
Non-thermal atmospheric-pressure plasma (NTAPP) is attracting widespread interest for use in medical applications. The tissue repair capacity of NTAPP has been reported in various fields; however, little is known about its effect on fracture healing. Non-union or delayed union after a fracture is a clinical challenge. In this study, we aimed to investigate how NTAPP irradiation promotes fracture healing in a non-union fracture model and its underlying mechanism, in vitro and in vivo. For the in vivo study, we created normal and non-union fracture models in LEW/SsNSlc rats to investigate the effects of NTAPP. To create a fracture, a transverse osteotomy was performed in the middle of the femoral shaft. To induce the non-union fracture model, the periosteum surrounding the fracture site was cauterized after a normal fracture model was created. The normal fracture model showed no significant difference in bone healing between the control and NTAPP-treated groups. The non-union fracture model demonstrated that the NTAPP-treated group showed consistent improvement in fracture healing. Histological and biomechanical assessments confirmed the fracture healing. The in vitro study using pre-osteoblastic MC3T3-E1 cells demonstrated that NTAPP irradiation under specific conditions did not reduce cell proliferation but did enhance osteoblastic differentiation. Overall, these results suggest that NTAPP is a novel approach to the treatment of bone fractures.
- Subjects
FRACTURE healing; UNUNITED fractures; ATMOSPHERIC pressure plasmas; NON-thermal plasmas; BONE fractures; ATMOSPHERIC pressure; TREATMENT of fractures
- Publication
PLoS ONE, 2024, Vol 19, Issue 4, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0298086