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- Title
Single-cell RNA-sequencing profiles reveal the developmental landscape of the Manihot esculenta Crantz leaves.
- Authors
Zang, Yuwei; Pei, Yechun; Cong, Xinli; Ran, Fangfang; Liu, Liangwang; Wang, Changyi; Wang, Dayong; Min, Yi
- Abstract
Cassava (Manihot esculenta Crantz) is an important crop with a high photosynthetic rate and high yield. It is classified as a C3–C4 plant based on its photosynthetic and structural characteristics. To investigate the structural and photosynthetic characteristics of cassava leaves at the cellular level, we created a single-cell transcriptome atlas of cassava leaves. A total of 11,177 high-quality leaf cells were divided into 15 cell clusters. Based on leaf cell marker genes, we identified 3 major tissues of cassava leaves, which were mesophyll, epidermis, and vascular tissue, and analyzed their distinctive properties and metabolic activity. To supplement the genes for identifying the types of leaf cells, we screened 120 candidate marker genes. We constructed a leaf cell development trajectory map and discovered 6 genes related to cell differentiation fate. The structural and photosynthetic properties of cassava leaves analyzed at the single cellular level provide a theoretical foundation for further enhancing cassava yield and nutrition. A single-cell fine map of cassava leaves reveals various types of cell characteristics and functions.
- Publication
Plant Physiology, 2024, Vol 194, Issue 1, p456
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1093/plphys/kiad500