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- Title
Identification of tomato F-box proteins functioning in phenylpropanoid metabolism.
- Authors
Shin, Doosan; Cho, Keun Ho; Tucker, Ethan; Yoo, Chan Yul; Kim, Jeongim
- Abstract
Phenylpropanoids, a class of specialized metabolites, play crucial roles in plant growth and stress adaptation and include diverse phenolic compounds such as flavonoids. Phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS) are essential enzymes functioning at the entry points of general phenylpropanoid biosynthesis and flavonoid biosynthesis, respectively. In Arabidopsis, PAL and CHS are turned over through ubiquitination-dependent proteasomal degradation. Specific kelch domain-containing F-Box (KFB) proteins as components of ubiquitin E3 ligase directly interact with PAL or CHS, leading to polyubiquitinated PAL and CHS, which in turn influences phenylpropanoid and flavonoid production. Although phenylpropanoids are vital for tomato nutritional value and stress responses, the post-translational regulation of PAL and CHS in tomato remains unknown. We identified 31 putative KFB-encoding genes in the tomato genome. Our homology analysis and phylogenetic study predicted four PAL-interacting SlKFBs, while SlKFB18 was identified as the sole candidate for the CHS-interacting KFB. Consistent with their homolog function, the predicted four PAL-interacting SlKFBs function in PAL degradation. Surprisingly, SlKFB18 did not interact with tomato CHS and the overexpression or knocking out of SlKFB18 did not affect phenylpropanoid contents in tomato transgenic lines, suggesting its irreverence with flavonoid metabolism. Our study successfully discovered the post-translational regulatory machinery of PALs in tomato while highlighting the limitation of relying solely on a homology-based approach to predict interacting partners of F-box proteins.Key message: Despite its highest sequence homology with Arabidopsis CHS-interacting KFB among 31 tomato KFBs, SlKFB18 does not function in CHS degradation, while predicted PAL-interacting SlKFBs function in PAL degradation in tomato.
- Publication
Plant Molecular Biology, 2024, Vol 114, Issue 4, p1
- ISSN
0167-4412
- Publication type
Article
- DOI
10.1007/s11103-024-01483-4