We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of three dental alloys on cytotoxicity and apoptosis related gene expression in L929 cells.
- Authors
MENG He; LI Xiu-mei; XU Yan-li; LIANG Rui-ying; WU Wen-hui
- Abstract
PURPOSE: To investigate the effect of the liching liquids of 3 different kinds of dental alloys on L929 cells both at cell level and molecular level. METHODS: The fibroblast L929 cells of mouse were treated in vitro with leaching liquids of 3 different kinds of dental alloys which were Au alloy, Ti and Ni-Cr alloy, respectively. The RPMI 1640 cell medium containing 10% fetal calf serum was served as a negative control and the Cu alloy was served as a positive control. The cytotoxicities of 3 dental alloys were evaluated by means of MTT and the effects of these alloys on the expression of caspase-3, caspase-8 and caspase-9 of L929 cells were examined by RT-PCR method and immunohistochemistry. The data was analyzed with SPSS 13.0 software package. RESULTS: The cytotoxicity of three groups were all in grade 0 after 48 hours of cultures. Caspase-8 had no change in all groups at mRNA level. The expression of caspase-3 and caspase-9 in Ni-Gr alloy group was significantly different from other groups. CONCLUSIONS: It is suggested from the results that the leaching liquids of Ni-Cr alloy may induce cell apoptosis through mitochondrion pathway.
- Subjects
DENTAL metallurgy; CELL-mediated cytotoxicity; APOPTOSIS; GENE expression; LEACHING; IN vitro studies; MITOCHONDRIAL physiology
- Publication
Shanghai Journal of Stomatology, 2013, Vol 22, Issue 1, p30
- ISSN
1006-7248
- Publication type
Article