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- Title
Effects of ADAM28 on biological functions of human dental pulp stem cells.
- Authors
ZHAO Zheng; LIU Hong-chen; Ling-ling, E.; WANG Yi; WANG Dong-sheng
- Abstract
PURPOSE: To investigate the effects of a disintegrin and metalloproteinase 28 (ADAM28) on proliferation, differentiation and apoptosis of human dental pulp stem cells (HDPSCs) and the possible mechanism. METHODS: Firstly, HDPSCs were isolated and cultured in vitro and identified. ADAM28 eukaryotic expression plasmid was constructed via gene rebuilt technique and transfected into HDPSCs. Then MTF chromatometry, enzyme dynamics and flow cytometry (FCM) techniques were performed to detect the effects of ADAM28 on biological characteristics of HDPSCs. Immunocytochemical and image analysis techniques were used to determine the influence of ADAM28 on HDPSCs expressing dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP) and osteopontin (OPN). Statisticalsignificance was assessed by the Student-Newman-Keuls (SNK) test with SPSS 13.0 software package. RESULTS: ADAM28 eukaryotic plasmid was constructed and transfected into HDPSCs for 48 hours successfully. In ADAM28 eukaryotic plasmid group, proliferation activity and index of HDPSCs were lower than those of pcDNA3.1 (+) group and untransfected group significantly. Alkaline phosphatase (ALP) secretion level and percentage of apoptotic cells went up remarkly. Significant difference was detected between eukaryotic plasmid group and other groups (P<0.05). The expression level of DSPP in HDPSCs elevated significantly (P<0.05). CONCLUSIONS: ADAM28 could inhibit HDPSCs proliferation, promote ALP secretion activity and DSPP expression in HDPSCs and induce HDPSCs apoptosis significantly. Supported by National Natural Science Foundation of China (Grant No. 30772450).
- Subjects
METALLOPROTEINASES; CELL differentiation; CELL proliferation; APOPTOSIS; DENTAL pulp; STEM cells
- Publication
Shanghai Journal of Stomatology, 2010, Vol 19, Issue 3, p262
- ISSN
1006-7248
- Publication type
Article