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- Title
Correlation between liver cell necrosis and circulating alanine aminotransferase after ischaemia/reperfusion injuries in the rat liver.
- Authors
Knudsen, Anders R.; Andersen, Kasper J.; Hamilton‐Dutoit, Stephen; Nyengaard, Jens R.; Mortensen, Frank V.
- Abstract
Circulating liver enzymes such as alanine transaminase are often used as markers of hepatocellular damage. Ischaemia/reperfusion (I/R) injury is an inevitable consequence of prolonged liver ischaemia. The aim of this study was to examine the correlation between liver enzymes and volume of liver cell necrosis after ischaemia/reperfusion injuries, using design-unbiased stereological methods. Forty-seven male Wistar rats were subjected to 1 h of partial liver ischaemia, followed by either 4 or 24 h of reperfusion. Within each group, one-third of animals were subjected to ischaemic preconditioning and one-third to ischaemic postconditioning. At the end of reperfusion, blood and liver samples were collected for analysis. The volume of necrotic liver tissue was subsequently correlated to circulating markers of I/R injury. Correlation between histological findings and circulating markers was performed using Pearson's correlation coefficient. Alanine transferase peaked after 4 h of reperfusion; however, at this time-point, only mild necrosis was observed, with a Pearson's correlation coefficient of 0.663 ( P = 0.001). After 24 h of reperfusion, alanine aminotransferase was found to be highly correlated to the degree of hepatocellular necrosis R = 0.836 ( P = 0.000). Furthermore, alkaline phosphatase ( R = 0.806) and α-2-macroglobulin ( R = 0.655) levels were also correlated with the degree of necrosis. We show for the first time that there is a close correlation between the volume of hepatocellular necrosis and alanine aminotransferase levels in a model of I/R injury. This is especially apparent after 24 h of reperfusion. Similarly, increased levels of alkaline phosphatase and α-2-macroglobulin are correlated to the volume of liver necrosis.
- Subjects
TREATMENT of reperfusion injuries; ALANINE aminotransferase; NECROSIS; ISCHEMIC preconditioning; ANIMAL models of ischemia; LIVER enzymes; LIVER cells; STEREOLOGY
- Publication
International Journal of Experimental Pathology, 2016, Vol 97, Issue 2, p133
- ISSN
0959-9673
- Publication type
Article
- DOI
10.1111/iep.12188