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- Title
In-vitro analysis of rhBMP-2 effects in human osteogenic cells.
- Authors
de Oliveira, Eder M.F.; Martinez, Elizabeth F.; Bossonaro, Jeruza P.; Ribeiro, Renato C.; de Araújo, Vera C.; Napimoga, Marcelo Henrique
- Abstract
This study evaluated the in vitro expression of bone-related proteins by osteoblasts in the presence of different concentrations of human recombinant bone morphogenetic protein-2 (rhBMP-2). Immortalized human fetal osteoblastic cell line 1.19 (hFOB) were exposed to different concentrations of rhBMP-2 (10, 50, or 100 ng/mL) for 72 h. Cell proliferation and viability (MTT assay), as well as the expression of fibronectin, osteonectin, and osteopontin were assessed by indirect immunofluorescence and Western blot. Neither of the 3 concentrations of rhBMP-2 caused statistically significant alterations in cell proliferation and viability, although the concentration of 100 ng/mL showed lower values for both assays after both 48 and 72 h of exposure. There was no alteration in the expression of noncollagenous proteins, as analyzed by immunofluorescence, when compared with the control group. Furthermore, in the Western blot assay we observed a statistically significant decrease in fibronectin and osteonectin at 100 ng rhBMP-2/mL ( p < 0.05) by comparison with the medium alone. The expression of osteopontin decreased slightly in all 3 concentrations of rhBMP-2 tested; however, the change was not statistically significant ( p > 0.05). In this in-vitro study, the tested concentrations of rhBMP-2 appeared to decrease the expression of important bone-related molecules in pre-osteoblast cells.
- Subjects
OSTEOBLASTS; CELL proliferation; IMMUNOFLUORESCENCE; OSTEOPONTIN; FIBRONECTINS; WESTERN immunoblotting
- Publication
Canadian Journal of Physiology & Pharmacology, 2013, Vol 91, Issue 11, p929
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/cjpp-2013-0080