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- Title
TNF Production from Peripheral Blood Mononuclear Cells in Diabetic Patients after Stimulation with Alginate and Lipopolysaccharide.
- Authors
Kulseng, B.; Skjåk-Bræk, G.; Følling, I.; Espevik, T.
- Abstract
Microencapsulated pancreatic Langerhan's islets in calcium alginate gels have been used us an implantable bio-artificial pancreas in the treatment of diabetes mellitus, but with limited success due to overgrowth of the capsule with fibroblasts and phagocytes. The authors earlier demonstrated that alginates enriched in mannuronic acid stimulate human monocytes to produce high levels of cytokines such as tumour necrosis factor (TNF), IL-1, IL-6. In this study the authors have measured the TNF production from peripheral blood mononuclear cells (PBMC) in different groups of insulin-dependent diabetes mellitus (IDDM) patients after stimulation with different alginates and lipopolysaccharide (LPS). It was found that high G-alginate did not induce TNF production in any of the groups. High-M alginate and LPS induced a dose-dependent TNF production in all groups and the production was significantly different from unstimulated cells. The highest TNF response was found in newly diagnosed IDDM patients and the lowest was in the controls.
- Subjects
DIABETES; CELLS; DIABETIC acidosis; ENDOCRINE diseases; CARBOHYDRATE intolerance; PHAGOCYTES; ALGINATES; MICROBIAL polysaccharides; CYTOKINES
- Publication
Scandinavian Journal of Immunology, 1996, Vol 43, Issue 3, p335
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.1996.d01-43.x