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- Title
Responses of Human T Cells to Dominant Discrete Protein Antigens of Escherichia coli and Pseudomonas aeruginosa.
- Authors
Kersten, C. M.; Mccluskey, R. T.; Shaw Warren, H.; Kurnick, J. T.
- Abstract
Normal human beings have circulating T lymphocytes that proliferate in response to <em>Escherichia coli</em> and <em>Pseudomonas aeruginosa</em>. We performed the present study to characterize the nature of the responding T cells and to determine whether distinct or shared conventional antigens, superantigens or polylonal activators account for T cell proliferation. Long term antigen-specific T cell lines were generated by repeated stimulation of PBMC from four donors with soluble antigen preparations of <em>E. coli</em> or <em>P. aeruginosa</em>. This resulted in the emergence of distinct T cell populations, which responded to strains of either <em>E. coli</em> or <em>P. aeruginosa</em>, but not to both. Trypsin treatment of the bacterial preparations largely eliminated their ability to stimulate the T cells. The T cell lines were predominantly CD4+ and their proliferation to bacterial antigens was optimal using autologous APC. <em>E. coli</em> T cell lines proliferated not only in response to the <em>E. coli</em> strain with which they were initially selected, but also to four different strains of <em>E. coli</em>, as well as to several related Gram-negative species. <em>P. aeruginosa</em> selected T cells exhibited proliferative responses to six different <em>P. aeruginosa</em> strains, but not to the other Gram-negative species. The finding that repeated stimulation of PBMC with <em>E. coli</em> or <em>P. aeruginosa</em> leads to CD4+ T cells highly reactive with conventional protein antigens specific either for <em>E. coli</em> or <em>P. aeruginosa</em> indicates that these bacteria possess separate dominant protein antigens that drive the proliferation of peripheral blood T cells.
- Subjects
T cells; PSEUDOMONAS aeruginosa; LYMPHOCYTES; ANTIGENS; CELL populations
- Publication
Scandinavian Journal of Immunology, 1994, Vol 40, Issue 2, p151
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1994.tb03444.x