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- Title
Interleukin-4 Down-Regulates Sendai Virus-Induced Production of Interferon-χ and -β in Human Peripheral Blood Monocytes In Vitro.
- Authors
Gobl, A. E.; Alm, G. V.
- Abstract
Recombinant inlerleukin-4(rIL-4) caused a 65-70% reduction of the interferon-α (IFN-α) and -β production induced by Sendai virus (SV) in human peripheral blood monocytes in vitro. Significant inhibition was seen at concentrations as low as 0.1 ng/ml. The rIL-4 also reduced levels of IFN-α and -β mRNA by 60% and 67%, respectively, as well as the frequency of IFN-α and -β mRNA-containing cells by 65% and 54%, respectively. The frequency of IFN-α/β mRNA-containing cells was inhibited by rIL-4 throughout the whole course of induction by SV. IL-4 caused a shift of the grain count distribution towards less heavily labelled cells, suggesting an inhibitory effect of rIL-4 on most IFN-α/β mRNA-containing cells. Antibodies to rlL-4 did not influence the normal IFN-α/β response induced by SV, but abolished the inhibitory effect of the rIL-4. When rIL-4 was added to cell 4 h after start of stimulation by SV, at which time much mRNA has accumulated but little IFN-α/β has been secreted, no inhibition of the IFN-α/β production by rIL-4 was seen. IFN-γ had only a minor reversing effect on the rIL-4 inhibition, but if cells were precultivated in medium with or without IFN-γ for 6 h before SV induction, rIL-4 paradoxically enhanced the IFN-α/β response. Our results suggest that rIL-4 inhibits an early step of IFN-α/β induction in monocytes, at the level either of transcription of IFN-α/β genes or of the processing or stability of mRNA. The IL-4 effects may however depend on the state of activation of the monocytes.
- Subjects
MESSENGER RNA; LEUCOCYTES; CELLS; SENDAI virus; ANTINEOPLASTIC agents; ANTIVIRAL agents
- Publication
Scandinavian Journal of Immunology, 1992, Vol 35, Issue 2, p167
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1992.tb02847.x