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- Title
Transduction of Mitogenic Signals in T Lymphocytes.
- Authors
Mustelin, T.; Pösö, H.; Ståhls, A.; Eloranta, T.; Andersson, Leif C.
- Abstract
Treatment of human T lymphocytes with mitogenic ligands. such as concanavalin A (Con A). induces a rapid activation of the enzyme ornithine decarboxylase (ODC). This activation occurs within minutes and is completely inhibited when the cells are treated with 1 mM Li* (in an inositol-free medium) prior to stimulation with Con A. In the presence of 1 mM myo-inositol Li* has no effect on the Con A-induced activation of ODC. To elucidate why inositol is needed for the mitogen-induced activation of ODC in T tymphocytes, we tested the ability of different inositol metabolites to reverse the inhibitory effect of Li+. Here we report that inositol phospholipids, in addition to inositol, reverse the Li*-induced inhibition of ODC activation, while all other inositol derivatives tested were ineffective. This indicates that Li+ does not block the activation of ODC by inhibiting the generation of inositol phosphates, but rather by a mechanism which is circumvented if inositol phospholipids are added. The molecular mechanisms involved in the rapid activation of ODC by mitogens in human T lymphocytes apparently require inositol phospholipids. but are not directly mediated by inositol-1.4,5-trisphosphate (IP3) alone, diacylglycerol alone, or other inositol phosphates.
- Subjects
T cells; GENETIC transduction; ORNITHINE decarboxylase; ENZYME activation; INOSITOL; VITAMIN B complex; MICROBIAL genetics; BIOLOGICAL products
- Publication
Scandinavian Journal of Immunology, 1987, Vol 26, Issue 3, p287
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1987.tb02262.x