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- Title
Isolation and Characterization of TR-c, an Antigen of the Reiter Treponeme Precipitating with Antibodies in Syphilis.
- Authors
Petersen, C. Sand; Pedersen, N. Strandberg; Axelsen, N. H.
- Abstract
TR-c is a Reiter treponemal antigen that cross-reacts with an antigen in Treponema pallidum (Nichols pathogenic strain). Sera from patients with secondary syphilis contain precipitating antibodies against TR-c. The isolation of TR-c from a crude bacterial sonicate involves five fractionation steps: anion exchange chromatography (DE-52 Whatman), gel filtration (Ac-A-22, Ultrogel), and affinity chromatography respectively on phenyl-Sepharose CL 4B, iminodiacetic acid-Sepharose CL 4B, and lysine-Sepharose 4B. The purified TR-c was enriched 320 times compared with the starting material, and the recovery was 22%. TR-c was shown to be a protein, it did not bind to a series of lectins, and by gel filtration and polyacrylamide gel electrophoresis (PAGE) the mol. wt was determined to be in the range of 630,000-730,000. It was found by SDS-PAGE to be composed of identical subunits, each having a mol. wt of 48,000. The isolated TR-c was immunochemically pure when tested in crossed immunoelectrophoresis against polyspecific anti-Reiter Ig. The purified TR-c antigen was used for production of a monospecific rabbit antiserum. Monospecific rabbit anti-TR-c gave strong fluorescence with both the Reiter treponeme and T. pallidum.
- Subjects
TREPONEMATOSES; CHROMATOGRAPHIC analysis; SEPHAROSE; GEL permeation chromatography; ZONE electrophoresis; POLYACRYLAMIDE gel electrophoresis
- Publication
Scandinavian Journal of Immunology, 1982, Vol 15, Issue 5, p459
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1982.tb00671.x