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- Title
Association between Single-Nucleotide Polymorphism (rs2072493) and Serum Level of TLR-5 and Interleukin-6 and Interleukin-12 Response to Toxoplasma gondii in Women with Miscarriage and Pregnant Women.
- Authors
Al-Baldawy, Adel N.; Al-Marsomy, Huda D.; Khaleel, Inas I.
- Abstract
Background: Toxoplasma gondii (T. gondii) is well-known to cause congenital diseases, or ocular diseases, and miscarriage in pregnancy. Earlier infections are threatening in pregnant women. The activity of Tolllike receptors (TLR) in defense against T. gondii infections was observed such as TLR-5 molecule, and some interleukins (like IL-6, and IL-12) that may lead to abortion. Objective: To study association between TLR-5 gene polymorphisms (rs2072493) with the susceptibility to toxoplasmosis in a sample of women with miscarriage and to study the association of TLR-5 polymorphisms (rs2072493) with the serum level of TLR-5, IL-6 and IL-12. Methods: This is a case-control study was conducted on 200 women, of which, 50 pregnant women seropositive (IgG, IgM) for T. gondii (Group1), 50 pregnant women seronegative (IgG, IgM) for T. gondii (Group2) as a control group, 50 women with miscarriage seropositive (IgG, IgM) for T. gondii (Group3), and 50 women with miscarriage seronegative (IgG, IgM) for T. gondii (Group4) as a control group. They were recruited from Balad General Hospital, Salah al-Din and private laboratory in the period from January 2021 to December 2021. The serum level of TLR-5, IL-6 and IL-12 were measured. The selected single-nucleotide polymorphism (SNPs) (rs2072493) in TLR-5 were detected by using real-time polymerase chain reaction with specific primers. Results: The frequency of the heterozygous genotype (TC) for SNPs TLR-5 gene (rs2072493) was significantly higher in Group1 than Group2 (P=0.004). At allelic level, the frequency of mutant allele (allele C) was significantly higher in Group1 than in Group2 (p=0.009), and in Group3 than in Group4 (P=0.025). There was significant increase in serum level of TLR-5 in Group1 and in Group3. There was significant increase in serum level of IL-6 and IL-12 in Group1. Conclusion: There is significant association of toxoplasmosis with mutant allele (allele C) of the SNP rs2072493, which may be considered as a risk factor for toxoplasmosis and stimulate miscarriage in pregnant women. TLR-5 level is high in both groups.
- Subjects
SINGLE nucleotide polymorphisms; PREGNANT women; RECURRENT miscarriage; INTERLEUKIN-12; TOXOPLASMA gondii; INTERLEUKIN-6
- Publication
Iraqi Journal of Medical Sciences, 2023, Vol 21, Issue 1, p124
- ISSN
1681-6579
- Publication type
Article
- DOI
10.22578/IJMS.21.1.14