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- Title
Isolation and Characterization of Arginase Producing Bacteria from Soil Compost Sites at Ogwa Community, Edo State, Nigeria.
- Authors
OKANLAWON, K. P.; OKANLAWON, T. S.; ADEDIRE, S. A.; AGBAJE, I. S.; ADEBOMI, O. H.; AKINBAMI, A. M.
- Abstract
Under typical physiological conditions, arginine plays a crucial function in the cell development and proliferation of healthy cells. Arginase has recently caught the attention of numerous researchers as a potential therapy option for auxotrophic cancer. Using standard morphological and biochemical methods, this paper isolates and characterize Arginase producing bacteria from soil compost sites collected from Ogwa community, Edo State, Nigeria. Enzymatic and physicochemical activities were assessed. The species identified to be present include Pseudomonas sp., Bacillus licheniformis, and Bacillus subtlis. The enzyme was active throughout a wide pH range, from 2 to 12. The optimal pH for the activity was found to be 9, and the ideal temperature was 70 °C. Km values for Arginine and Guanidine hydrochloride were 0.21 mM and 0.25 mM, respectively, in terms of substrates. Studies on the enzyme with different cations showed that the activity of the enzyme was affected by Sn2+, Hg2+, Pb2+, Na2+, and K+. This study shows the therapeutically significant arginase enzyme is present in the isolated bacteria from various compost sites.
- Subjects
EDO State (Nigeria); CAPE Coast (Ghana); NIGERIA; ARGINASE; SOIL microbiology; COMPOSTING; BACILLUS licheniformis; GUANIDINIUM chlorides
- Publication
Journal of Applied Sciences & Environmental Management, 2024, Vol 28, Issue 5, p1443
- ISSN
1119-8362
- Publication type
Article
- DOI
10.4314/jasem.v28i5.15