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- Title
Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas.
- Authors
Pateras, Ioannis S.; Evangelou, Konstantinos; Tsimaratou, Katerina; Liontos, Michalis; Sakellariou, Stratigoula; Barlogiannis, Theodoros; Karakitsos, Petros; Papalois, Apostolos; Kotsinas, Athanassios; Gorgoulis, Vassilis G.
- Abstract
The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCRRestriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiacmyxomas, whereas no HSVDNA was detected in normal heart tissues (P < 0.01). The existence of HSV-1/2DNA in sporadic cardiacmyxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.
- Subjects
HERPESVIRUS disease genetics; CELL culture; FISHER exact test; HEART tumors; HERPESVIRUS diseases; MYXOMA; POLYMERASE chain reaction; RESEARCH funding; U-statistics; DESCRIPTIVE statistics; SEQUENCE analysis; DISEASE complications
- Publication
Journal of Biomedicine & Biotechnology, 2012, Vol 2012, p1
- ISSN
1110-7243
- Publication type
Article
- DOI
10.1155/2012/823949