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- Title
Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity.
- Authors
Yang, Wanjie; Tang, Qiguo; Bai, Ying; Wang, Kejun; Dong, Xinxing; Li, Ying; Fang, Meiying
- Abstract
Bacterial magnetic particles (BMPs) are biosynthesized magnetic nano-scale materials with excellent dispersibility and biomembrane enclosure properties. In this study, we demonstrate that BMPs augment the ability of polyethylenimine (PEI) to deliver target DNA into difficult-to-transfect primary porcine liver cells, with transfection efficiency reaching over 30%. Compared with standard lipofection and polyfection, BMP-PEI gene vectors significantly enhanced the transfection efficiencies for the primary porcine liver cells and C2C12 mouse myoblast cell lines. To better understand the mechanism of magnetofection using BMP-PEI/DNA vectors, transmission electron microscopy (TEM) images of transfected Cos-7, HeLa, and HEP-G2 cells were observed. We found that the BMP-PEI/DNA complexes were trafficked into the cytoplasm and nucleus by way of vesicular transport and endocytosis. Our study builds support for the versatile BMP-PEI vector transfection system, which might be exploited to transfect a wide range of cell types or even to reach specific targets in the treatment of disease. Key Points: • We constructed a BMP-PEI gene delivery vector by combining BMPs and PEI. • The vector significantly enhanced transfection efficiencies in eukaryotic cell lines. • The transfection mechanism of this vector was explained in our study.
- Subjects
MYOBLASTS; LIVER cells; MAGNETIC particles; EUKARYOTIC cells; TRANSMISSION electron microscopy; MAGNETIC materials
- Publication
Applied Microbiology & Biotechnology, 2020, Vol 104, Issue 15, p6799
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-020-10729-2