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- Title
Cloning of a novel gene involved in alkane biosynthesis from Klebsiella sp.
- Authors
Ito, Masakazu; Kambe, Hiromi; Sawagashira, Ai; Kishino, Shigenobu; Takeuchi, Michiki; Ando, Akinori; Muramatsu, Masayoshi; Ogawa, Jun
- Abstract
Aliphatic medium-chain alkanes, a major component of gasoline, diesel, and jet fuels, are drop-in compatible fuels. Microorganisms with the capacity to produce medium-chain alkanes are promising for the bio-production of drop-in fuel. We found that Klebsiella sp. NBRC100048 has the ability to produce medium-chain alkanes from medium-chain aldehydes. We cloned a gene involved in conversion of aldehydes to alkanes by using a genomic fosmid library derived from Klebsiella sp. NBRC100048. The gene termed orf2991 encodes 506 amino acids and shows 62% sequence homology to the aldehyde dehydrogenase of Escherichia coli, aldB. The finding of orf2991 as a novel alkane-synthesizing enzyme gene similar to E. coli aldehyde dehydrogenase family, which is generally known to catalyze a reaction oxidizing aldehydes to fatty acids, indicated a novel function of aldehyde dehydrogenase. This finding is not only significant academically but allows developing the novel manufacturing methods of alkanes fermentation.
- Subjects
ALDEHYDE dehydrogenase; KLEBSIELLA; ALKANES; GENE conversion; BIOSYNTHESIS; MOLECULAR cloning
- Publication
Applied Microbiology & Biotechnology, 2019, Vol 103, Issue 14, p5917
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-019-09900-1