We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
4-Hydroxybenzaldehyde sensitizes <italic>Acinetobacter baumannii</italic> to amphenicols.
- Authors
Shin, Bora; Park, Chulwoo; Imlay, James A.; Park, Woojun
- Abstract
Bacterial metabolism modulated by environmental chemicals could alter antibiotic susceptibility. 4-Hydroxybenzaldehyde (4-HBA), which cannot support the growth of <italic>Acinetobacter baumannii</italic>, exhibited synergism only with amphenicol antibiotics including chloramphenicol (CAM) and thiamphenicol. Interestingly, this synergistic effect was not observed with other growth-supporting, structurally similar compounds such as 4-hydroxybenzoate. Transcriptomic analysis demonstrated that genes involved in protocatechuate metabolism (<italic>pca</italic> genes) and osmotic stress (<italic>bet</italic> genes) were significantly upregulated by 4-HBA and CAM treatment. The 14C-labeled CAM influx was lower in a <italic>pcaK1</italic> (encoding a transporter of protocatechuate) deletion mutant and was higher in the <italic>pcaK1</italic> overexpressing cells relative to that in the wild type upon 4-HBA treatment. Our kinetic data using 14C-labeled CAM clearly showed that CAM uptake is possibly through facilitated diffusion. Deletion of <italic>pcaK1</italic> did not result in the elimination of CAM influx, indicating that CAM does not enter only through PcaK1. The amount of 4-HBA in the culture supernatant was, however, unaffected during the test conditions, validating that it was not metabolized by the bacteria. CAM resistant <italic>A. baumannii</italic> cells derived by serial passages through CAM-amended media exhibited lower level of <italic>pcaK1</italic> gene expression<italic>.</italic> These results led us to conclude that the activation of PcaK1 transporter is probably linked to cellular CAM susceptibility. This is the first report showing a relationship between CAM influx and aromatic compound metabolism in <italic>A. baumannii</italic>.
- Subjects
ACINETOBACTER baumannii; ENVIRONMENTAL chemistry; BENZALDEHYDE; THIAMPHENICOL; DRUG synergism
- Publication
Applied Microbiology & Biotechnology, 2018, Vol 102, Issue 5, p2323
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-018-8791-1