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- Title
In vitro Investigation of the Effects of Hylotelephium and Typha Plant Extracts on Anti-inflammatory and Wound Healing.
- Authors
GÜLER, Ece; BABALI BALIBEY, Fatmanur; ÖZMAN, Zeynep; BAŞARICI, Mehmet Timur; KOÇYİĞİT, Abdurrahim
- Abstract
Introduction: Hylotelephium and Typha species are known for their widespread use for treating wounds. This study investigated the total phenolic and flavonoid content, antioxidant capacities, wound healing-inducing and anti-inflammatory activity of these two species. Method: After preparation of Typha domingensis (TDME) and Hylotelephium spectabile methanolic extracts (HSME), total phenol, flavonoid content and antioxidant activity were determined. Cell viability and the optimal doses for the scratch assay for HaCat and CCD-1072 cells were evaluated by MTT assay. The migration abilities of cells were evaluated using the scratch assay and analyzed by ImageJ software. Results: The total phenolic content of HSME and TDME was found to be 9.8 and 36.8 mgGallicacid Eq/g, while the total flavonoid content of HSME and TDME were 6.3 and 13.8 mgQueEq/g, respectively. Also, the antioxidant capacities of HSME and TDME were found to be 312.9 and 521.4 mg ascorbicacidEq/g, respectively. The maximum non-toxic dose for extracts was determined as 200 µg/mL, according to the MTT results. The maximum percentage of wound closure area with HaCaT cells after 16 h was 85.28% for 10 ug/mL TDME and 89.55% for 200 ug/mL HSME. However, after 24 h, 50 ug/mL TDME showed 91.45% area closure, while 200 ug/mL HSME showed 93.42%. Also, with the fibroblasts, it was observed that 43.27% and 61.37% of the wound area were closed after 16 and 24 h for 100 ug/mL TDME, while the control group exhibited 22.29% and 35.89% area closure, respectively. Conclusion: TDME was found to have higher phenolic and flavonoid content and antioxidant capacity. In conclusion, the findings of this study provide significant evidence for the presence of wound-healing properties in both HSME and TDME.
- Subjects
PLANT extracts; TYPHA; HEALING; FLAVONOIDS; OXIDANT status; CELL migration inhibition
- Publication
Bezmialem Science, 2023, Vol 11, pS4
- ISSN
2148-2373
- Publication type
Abstract