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- Title
Mass spectrometry as a novel method for detection of podocyturia in pre-eclampsia.
- Authors
Garovic, Vesna D.; Craici, Iasmina M.; Wagner, Steven J.; White, Wendy M.; Brost, Brian C.; Rose, Carl H.; Grande, Joseph P.; Barnidge, David R.
- Abstract
Background Podocyturia, i.e. urinary loss of viable podocytes, may serve as a diagnostic tool for pre-eclampsia and as a marker of active renal disease. The current method to detect podocyturia is technically complex, lengthy and requires a high level of expertise for interpretation. The aim of this study was to develop a new technique for the identification of urinary podocytes, based on the detection of podocyte-specific tryptic peptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), which will provide an operator-independent and highly reproducible method. Methods and Results The diagnosis of pre-eclampsia was confirmed in the presence of hypertension (>140/90 mmHg) and proteinuria >0.3 g/24 h urine. The diagnosis of HELLP was confirmed based on the accepted clinical criteria of hemolysis, elevated liver enzymes and low platelet count. Conclusion The LC-MS/MS method is a reliable technology for the identification of urinary podocytes, based on the presence of podocyte-specific proteins in the urine.
- Subjects
PROTEINURIA diagnosis; HYPERTENSION in pregnancy; URINALYSIS; LIQUID chromatography-mass spectrometry; HEMOLYSIS &; hemolysins; BIOMARKERS
- Publication
Nephrology Dialysis Transplantation, 2013, Vol 28, Issue 6, p1555
- ISSN
0931-0509
- Publication type
Article
- DOI
10.1093/ndt/gfs074