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- Title
Preliminary Exposure to Histone Deacetylase Inhibitors Changes the Direction of Human iPSCs' Differentiation with the Formation of Cardiospheres Instead of Skin Organoids.
- Authors
Abdyev, V. K.; Riabinin, A. A.; Erofeeva, E. D.; Pankratova, M. D.; Vorotelak, E. A.; Vasiliev, A. V.
- Abstract
Pluripotent stem cells (PSCs) are a unique cell type that can differentiate into all cell types in the body. In PSC culture, subpopulations with different levels of pluripotency may exist, which leads to different results during their differentiation. One of the key factors that determine the state of pluripotency and influence the differentiation potential of PSCs is the epigenetic state of cells, including the level of histone deacetylation. Activation of histone deacetylase (HDAC) in human and mouse PSCs increases the percentage of heterochromatin. In this work, we used a protocol for the differentiation of embryoid bodies from induced human pluripotent hiPSC cells designed for the formation of ectoderm and neuroectoderm with their subsequent development into skin organoids. However, after hiPSCs were exposed to HDAC inhibitors (sodium butyrate and valproic acid), the direction of their differentiation changed: mesoderm was formed, which subsequently developed into contracting cardiospheres.
- Subjects
HISTONE deacetylase inhibitors; HYDROXAMIC acids; BUTYRATES; PLURIPOTENT stem cells; SODIUM butyrate; HISTONE deacetylase; ORGANOIDS
- Publication
Russian Journal of Developmental Biology, 2023, Vol 54, Issue 6, p350
- ISSN
1062-3604
- Publication type
Article
- DOI
10.1134/S1062360423060024