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- Title
The seed endosphere of <italic>Anadenanthera colubrina</italic> is inhabited by a complex microbiota, including <italic>Methylobacterium</italic>spp. and <italic>Staphylococcus</italic> spp. with potential plant-growth promoting activities.
- Authors
Alibrandi, Pasquale; Cardinale, Massimiliano; Rahman, MD Mahafizur; Strati, Francesco; Ciná, Paolo; de Viana, Marta L.; Giamminola, Eugenia M.; Gallo, Giuseppe; Schnell, Sylvia; De Filippo, Carlotta; Ciaccio, Mirella; Puglia, Anna Maria
- Abstract
Background and aims: Plant seeds are emerging micro–habitats, whose importance as reservoir and vector of beneficial microbes just begins to be recognized. Here we aimed to characterize the bacterial microbiota of the <italic>Anadenanthera colubrina</italic> seed endosphere, with special focus to beneficial traits and to the colonization pattern.Methods: Cultivation–dependent (isolation from surface–sterilized seeds) and cultivation–independent (pyrosequencing of 16S rRNA gene from metagenomic seed DNA) analyses, functional tests and microscopical investigations (fluorescence <italic>in situ</italic> hybridization coupled with confocal laser scanning microscopy (FISH-CLSM) were performed.Results: We isolated several <italic>Methylobacterium</italic> and <italic>Staphylococcus</italic> spp., exhibiting both plant growth promotion and antimicrobial activities. The two taxonomic groups showed complementary traits, which supports a functional selection. Both genera were detected also by pyrosequencing, together with further taxa. The genera <italic>Friedmaniella</italic>, <italic>Bifidobacterium, Delftia</italic>, <italic>Anaerococcus</italic> and <italic>Actinomyces</italic> appeared here for the first time as seed endophytes. We detected bacterial cells and micro–colonies in seed cryosections by FISH-CLSM. Alphaproteobacteria, Firmicutes and other bacteria colonized intercellular spaces of the parenchyma and associated to transport vessels.Conclusions: This work sheds light onto the diversity, functions and colonization pattern of the <italic>Anadenanthera colubrina</italic> seed endophytes, and strongly suggest a role as beneficial partners for seed-associated microbiota.
- Subjects
PLANT-microbe relationships; SEED microbiology; PLANT growth; PYROSEQUENCING; FLUORESCENCE in situ hybridization; CONFOCAL fluorescence microscopy
- Publication
Plant & Soil, 2018, Vol 422, Issue 1/2, p81
- ISSN
0032-079X
- Publication type
Article
- DOI
10.1007/s11104-017-3182-4