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- Title
RESISTANT STRAINS OF ESCHERICHIA COLI IN THE ENVIRONMENT OF THE MARTIN REGION COMPARED TO RESISTANT STRAINS IN SLOVAKIA.
- Authors
Tatiana, HAVRILOVÁ; Elena, NOVÁKOVÁ; Vladimíra, SADLOŇOVÁ
- Abstract
Introduction: This paper concerns testing for microorganisms with antimicrobial resistance (AMR) in the urban environment, focusing on the samples of microorganisms that can transiently colonise the human gut microbiome from children's sandpits. Aim: The aim was to diagnose resistant Escherichia coli strains from sandpits in the Martin region in 2018. The findings were compared with other data on resistance in Escherichia coli in Slovakia gathered between 2017 and 2021. Methods: Saline solutions were prepared from the sandpit samples, which were then cultivated on 3 different agars (Endo agar, deoxycholate-citrate agar and HiChrome agar for Escherichia coli). Analysis with MALDI-TOF (matrix assisted laser desorption/ionization - time of flight) mass spectrometry confirmed the presence of various bacterial species in the samples including Escherichia coli. The antimicrobial susceptibility of the E. coli strains was determined using a disc diffusion method and a quantitative method establishing a minimum inhibitory concentration. Results: A total of 32 different bacterial species were identified in the sand samples, of which the most numerous species was Escherichia coli. Three of the strains of E. coli isolated from the environment were resistant to multiple antibiotics (aminopenicillins, fluoroquinolones, tetracyclines) at time when other research found only a slight decrease in resistance to four types of antibiotics in Slovakia (aminopenicillins, fluoroquinolones, third generation cephalosporins and aminoglycosides). A high percentage of resistance (50 - 70 %) persists to the present at least in the case of aminopenicillins. Conclusion: The reported findings of the presence of resistant E. coli strains in an urban environment indicate that it would be beneficial to include playgrounds in monitoring and to conduct regular testing to track the changes in resistance.
- Subjects
SLOVAKIA; ESCHERICHIA coli; GUT microbiome; DRUG resistance in microorganisms; ANTIBIOTICS
- Publication
Zdravotnicke listy, 2023, Vol 11, Issue 4, p44
- ISSN
1339-3022
- Publication type
Article