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- Title
FRACTIONATION AND CHARACTERIZATION OF PROTEINS OF SOME EGYPTIAN FIELD CROPS.
- Authors
Nofe1, Zakaria A.; Taha, M. G.; Ali, H. E.; Darwish, N. N.
- Abstract
The proteins considered a true expression of genetic characteristics in plant crops and can be used for classifying of many varieties of different crops by using protein electrophoreses SDS PAGE, to fractionate and characterize proteins. Results showed that barley crop whose varieties had a protein C-hordein with molecular weight 21 KDa which characterized in each variety but differ in its percentage. Com crop varieties were characterized by presence of zien protein with molecular weight 19, 17 and l lKDa. While in rice crop, varieties :were differed according to protein bands especially in tublin protein with molecular weight 48KDa. Also, the varieties of wheat crop were differed by percentage of some essential protein glia4in: gamma-beta-alpha, with molecular weight 58,42 and 35KDa, respectively. On the other hand, legumes crops: chickpea, faba bean and lentil proteins could be characterized with a lot of protein bands and had been noticed greet difference in kinds of protein or in its percentage of different varieties through molecular weight from 245KDa (Gamma globulin protein) to l IKDa Vicilins proteins. While in fiber crops proteins: cotton and flax were had a less protein bands which were appeared in some varieties of flax protein whose had one protein band (conlinin) with molecular weight 18KD, also cotton protein varieties had different percentages of 7S vicilins proteins with molecular weight 49 and 46KDa. Although oil crops: peanuts, soybean and sunflower proteins had clear differences in all varieties as number of protein bands and/or percentages. Peanuts varieties had the same protein bands their weights from 88KDa (glutenin) to 19KDa (Conarachin) but as a different percentages in each variety. In soybean proteins varieties there were differences in number of protein bands and percentages, sunflower varieties had almost the same protein bands but differed in their percentages and their weights were between 245KDa to l lKDa. Results confirmed that protein electrophoreses could be used as a simple method of classification of filed crop varieties.
- Subjects
PROTEIN fractionation; FIELD crops; LEGUMES; WHEAT; FAVA bean; SOY proteins
- Publication
Zagazig Journal of Agricultural Research, 2019, Vol 46, Issue 2, p475
- ISSN
1110-0338
- Publication type
Article
- DOI
10.21608/zjar.2019.33402