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- Title
Tissue culture and plant regeneration from Torenia biniflora.
- Authors
WANG Ying-Hua; SHI Qiu-Ying; CHEN Xiong-Wei; CHEN Gang; JIN Hong
- Abstract
Torenia biniflora is a member of Scrophulariaceae Torenia , and it is an annual flowering plant that widely grows in Chinese subtropical area, such as Guangdong Province and Guangxi Zhuang Autonomous Region. T. biniflora is a wild flower with high great ornamental value. However, in natural growth condition, the reproduction speed of T. Biniflora is slow, proliferation rate is low, and the kinds of flower colour and type are few, therefore, it is unable to meet the diversified need of market. Plant tissue culture technology provides a new way to improve and breed new varieties for ornamental plants. Up to now, a lot of members of Torenia plants have been successfully established in vitro regeration system through tissue culture, such as T. fournieri Linden, T. baillonii Susie Wong and T. concolor Lindl. ,etc. While,tissue culture for T. Biniflora has never been reported. The main objective of this research was to establish in vitro regeneration system of T. Biniflora through tissue culture and study the effects of plant growth substances such as IBA, 6-BA and NAA for clonal multiplication. In this study, we created a reliable protocol for regenerating T. biniflora plants in tissue culture by using leaf explants. The effects of different concentrations and combinations of plant growth substances on adventitious bud induction and growth were studied, and the effects of different ionic strengths of media and concentrations of IBA on rooting were analyzed. According to a series of indices, such as average number of buds, frequency of bud induction, frequency of rooting, average number of root and average length of root, the optimal media for inducing adventitious buds and rooting were screened respectively. The results showed that adventitious bud induction was related with the concentrations and combinations of plant growth regulator, and the optimal medium for buds induction was MS+ 6-BA 0.5 mg • L-1 + NAA 0.2 mg • L-1. The best medium for rooting was 1/2 MS as basal medium with IBA 0.05 mg • L-1. In this study the in vitro plant reproductive system of T. biniflora with high frequence was completely established, and it would provide an important experiment foundation for the research of rapid propagation and genetic transformation for T. biniflora.
- Publication
Guihaia, 2015, Vol 35, Issue 2, p150
- ISSN
1000-3142
- Publication type
Article
- DOI
10.11931/guihaia.gxzw201307019