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- Title
Skeletal muscle interleukin-6 regulates metabolic factors in i WAT during HFD and exercise training.
- Authors
Knudsen, Jakob G.; Bertholdt, Lærke; Joensen, Ella; Lassen, Signe B.; Hidalgo, Juan; Pilegaard, Henriette
- Abstract
Objective To investigate the role of skeletal muscle (SkM) interleukin (IL)-6 in the regulation of adipose tissue metabolism. Methods Muscle-specific IL-6 knockout (IL-6 MKO) and IL-6loxP/loxP (Floxed) mice were subjected to standard rodent diet (Chow), high-fat diet (HFD), or HFD in combination with exercise training (HFD ExTr) for 16 weeks. Results Total fat mass increased ( P < 0.05) in both genotypes with HFD. However, HFD IL-6 MKO mice had lower ( P < 0.05) inguinal adipose tissue (iWAT) mass than HFD Floxed mice. Accordingly, iWAT glucose transporter 4 (GLUT4) protein content, 5′AMP activated protein kinase (AMPK)Thr172 phosphorylation, and fatty acid synthase (FAS) mRNA content were lower ( P < 0.05) in IL-6 MKO than Floxed mice on Chow. In addition, iWAT AMPKThr172 and hormone-sensitive lipase (HSL)Ser565 phosphorylation as well as perilipin protein content was higher ( P < 0.05) in HFD IL-6 MKO than HFD Floxed mice, and pyruvate dehydrogenase E1α (PDH-E1α) protein content was higher ( P < 0.05) in HFD ExTr IL-6 MKO than HFD ExTr Floxed mice. Conclusions These findings indicate that SkM IL-6 affects iWAT mass through regulation of glucose uptake capacity as well as lipogenic and lipolytic factors.
- Subjects
INTERLEUKIN-6; SKELETAL muscle; ADIPOSE tissues; PROTEIN kinases; HIGH-fat diet; LABORATORY mice
- Publication
Obesity (19307381), 2015, Vol 23, Issue 8, p1616
- ISSN
1930-7381
- Publication type
Article
- DOI
10.1002/oby.21139