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- Title
Preparation of Monoclonal Antibody against Pyrene and Benzo [a]pyrene and Development of Enzyme-Linked Immunosorbent Assay for Fish, Shrimp and Crab Samples.
- Authors
Wu, Shuangmin; Li, Huaming; Yin, Xiaoyang; Si, Yu; Qin, Liangni; Yang, Hongfei; Xiao, Jiaxu; Peng, Dapeng
- Abstract
Polycyclic aromatic hydrocarbons (PAHs) are significant environmental and food pollutants that can cause cancer. In this work, a specific monoclonal antibody (mAb) to identify pyrene (PYR) and benzo [a]pyrene (BaP) was prepared, and an indirect competitive enzyme-linked immunoassay (ic-ELISA) was established to detect PYR and BaP residues in living aquatic products for the first time. The effects of complete antigens with different coupling ratios on the production of high-sensitivity mAb was explored. Under the optimal conditions, the IC50 value was 3.73 ± 0.43 µg/L (n = 5). The limits of detection (LODs) for PYR and BaP in fish, shrimp, and crab ranged from 0.43 to 0.98 µg/L. The average recoveries of the spiked samples ranged from 81.5–101.9%, and the coefficient of variation (CV) was less than 11.7%. The validation of the HPLC-FLD method indicated that the ELISA method set up in this experiment provided a trustworthy tool for PAHs residues detection in aquatic products.
- Subjects
BENZOPYRENE; PYRENE; POLLUTANTS; POLYCYCLIC aromatic hydrocarbons; MONOCLONAL antibodies; SHRIMPS; ENZYME-linked immunosorbent assay
- Publication
Foods, 2022, Vol 11, Issue 20, p3220
- ISSN
2304-8158
- Publication type
Article
- DOI
10.3390/foods11203220