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- Title
A De-O-acylated Lipooligosaccharide-Based Adjuvant System Promotes Antibody and Th1-Type Immune Responses to H1N1 Pandemic Influenza Vaccine in Mice.
- Authors
Ryu, Ji In; Park, Shin Ae; Wui, Seo Ri; Ko, Ara; Han, Ji Eun; Choi, Jung Ah; Song, Man Ki; Kim, Kwang Sung; Cho, Yang Je; Lee, Na Gyong
- Abstract
Vaccine adjuvants are agents that are used to promote immune responses to vaccine antigens and thereby to enhance the protective efficacy of the vaccines. In this study, we investigated the adjuvant activity of CIA06, an adjuvant system that is composed of a toll-like receptor 4 agonist de-O-acylated lipooligosaccharide (dLOS) and aluminum hydroxide, on the H1N1 pandemic influenza vaccine Greenflu-S® in mice. CIA06 significantly enhanced influenza-specific serum IgG, hemagglutination-inhibition, and virus-neutralizing antibody titers, which eliminated vaccine dose-dependency in the antibody response. Mice immunized with the CIA06-adjuvanted Greenflu-S showed Th1-type-predominant cytokine profiles, and both CD4+ and CD8+ T cell responses were induced. Immunization of mice with the CIA06-adjuvanted vaccine reduced the mortality and morbidity of mice upon lethal challenges with influenza virus, and no excessive inflammatory responses were observed in the lung tissues of the immunized mice after viral infection. These data suggest that the dLOS-based adjuvant system CIA06 can be used to promote the immune responses to influenza vaccine or to spare antigen dose without causing harmful inflammatory responses.
- Subjects
IMMUNOLOGICAL adjuvants; ANALYSIS of variance; ANIMAL experimentation; CYTOKINES; INFLUENZA vaccines; MICE; PROBABILITY theory; RESEARCH funding; SURVIVAL; REPEATED measures design; H1N1 influenza; DATA analysis software; KAPLAN-Meier estimator; ANTIBODY formation; LOG-rank test; ONE-way analysis of variance; THERAPEUTICS
- Publication
BioMed Research International, 2016, Vol 2016, p1
- ISSN
2314-6133
- Publication type
Article
- DOI
10.1155/2016/3713656