We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin.
- Authors
HANZHANG DONG; GAOGAO LIU; BIAO JIANG; JIUBING GUO; GUOQUAN TAO; WEI YIU; JINGSONG ZHOU; GUOXIN LI
- Abstract
The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long-term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/CDDP and SGC7901 cells were examined by reverse transcription polymerase chain reaction and western blotting respectively. The results revealed morphological differences between the SGC7901 and SGC7901/CDDP cells. The expression levels of Survivin mRNA and protein were significantly higher in the SGC7901/CDDP cells than in the SGC7901 cells. Therefore, high expression levels of the Survivin gene may explain SGC7901 cell resistance to CDDP.
- Subjects
SURVIVIN (Protein); GENE expression; MOLECULAR genetics; CISPLATIN; GENETIC regulation
- Publication
Oncology Letters, 2014, Vol 8, Issue 5, p1953
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2014.2463