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- Title
DISTINCTIVE LECTIN LABELING OF THE FLAGELLAR APPARATUS OF <em>TETRASELMIS</em> (PRASINOPHYCEAE) AND <em>DUNALIELLA</em> (CHLOROPHYCEAE).
- Authors
Costas, Eduardo; Hevia, Elena; Carretero, Yolanda; López-Rodas, Victoria
- Abstract
Fluorescent labeling of the flagellar apparatus of Tetraselmis (Prasinophyceae) and Dunaliella (Polyble-pharidaceae, Chlorophyceae) were successfully performed using fluorescein isothiocyanate-labeled lectins from arachis hypogae and Glycine maxima. These lectins specifically bound to the flagella and kinetosome of the cell but did not bind to the cell surface. Lectin binding on the flagellar apparatus remained constant under different culture media, temperatures, irradiances, cell division cycle, and culture aging. All the Tetraselmis and Dunaliella analyzed (five species, 20 clones) showed intense labeling of the flagellar apparatus. In contrast, no other species analyzed (46 clones of 25 species from four classes) showed binding to their flagellar apparatus. After the lectin treatment, many cells remained alive, and they were able to swim with the flagellar apparatus intensely labeled. The lectin binding indicates that the flagella and kinetsome of Tetraselmis are rich in Gal and Gal NH[sub2] moieties and that the flagella of Dunaliella are rich in Gal and Gal Nac moieties. Apparently, this feature seems to be specific to these species.
- Subjects
DUNALIELLA; GREEN algae; LECTINS; IMMUNOGLOBULINS; TETRAS; POLYBLEPHARIDACEAE
- Publication
Journal of Phycology, 1996, Vol 32, Issue 5, p847
- ISSN
0022-3646
- Publication type
Article
- DOI
10.1111/j.0022-3646.1996.00847.x