We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease.
- Authors
Pilkington, L.J.; Gibb, K.S.; Gurr, G.M.; Fletcher, M.J.; Nikandrow, A.; Elliott, E.; van de Ven, R.; Read, D.M.Y.
- Abstract
Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellows-affected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out.
- Subjects
AUSTRALIA; ALFALFA diseases &; pests; SEED crops; PLANT diseases; LEGUME diseases &; pests; PLANT genetics
- Publication
Plant Pathology, 2003, Vol 52, Issue 6, p754
- ISSN
0032-0862
- Publication type
Article
- DOI
10.1111/j.1365-3059.2003.00934.x