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- Title
Sleep prolonging impact of fractions of Ocimum basilicum extract in pentobarbital-induced sleep in mice and possible mechanism.
- Authors
Rahimi, Vafa Baradaran; Askari, Vahid Reza; Ghorbani, Ahmad; Rakhshandeh, Hassan
- Abstract
Objectives: Sleep disorders are accompanied by several complications and currently used soporific drugs can induce unwanted effects such as psychomotor impairment, tolerance, amnesia, and rebound insomnia. Materials and Methods: This work was an experimental study on 56 mice which were randomly divided into 7 groups: saline (control); diazepam (3 mg/kg, positive control), fractions of hydro ethanolic extract of Ocimum basilicum including: ethyl acetate fraction (EAF, 50 mg/kg); n-butanol fraction (NBF, 50 mg/kg); water fraction (WF, 50 mg/kg); and saline containing 10% DMSO (vehicle for EAF and NBF) and flumazenil (1mg/kg). All test compounds were injected intraperitoneally 30 min before pentobarbital administration (30 mg/kg). Duration and latency of pentobarbital-induced sleep were recorded. Also, cytotoxicity of fractions was tested on neural and fibroblast cells using MTT assay. Results: The hypnotic effect of fractions was comparable to that induced by diazepam. WF, EAF and NBF at 50 mg/kg could increase sleep duration (p<0.001). The sleep latency was decreased by NBF (p<0.001), but not by WF and EAF. Also, flumazenil could significantly reverse hypnotic effect of NBF (p<0.001). None of the fractions had any significant effects on viability of neuronal PC12 cells and L929 fibroblast cells. Conclusion: The present data demonstrated that Ocimum basilicum fractions potentiate sleeping behaviors without any cytotoxicity. The main component(s) responsible for the hypnotic effects of this plant is most likely non-polar agent(s) found in NBF. Moreover, NBF acts on neurons and sleep by GABA receptors respects to flumazenil.
- Subjects
BASIL; SLEEP disorders; LABORATORY rats
- Publication
Avicenna Journal of Phytomedicine, 2015, Vol 5, p125
- ISSN
2228-7930
- Publication type
Article