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- Title
Thermostable and organic solvent-tolerant acid pectinase from Aspergillus terreus FP6: purification, characterization and evaluation of its phytopigment extraction potential.
- Authors
Bhattacharyya, Rajrupa; Mukhopadhyay, Dibbyangana; Nagarakshita, V. K.; Bhattacharya, Sourav; Das, Arijit
- Abstract
The present study discusses the purification, characterization and application of pectinase from Aspergillus terreus FP6 in fruit pigment extraction. By the four-step purification involving precipitation, dialysis, ion-exchange chromatography, gel filtration chromatography, a 20.85-fold purification of the enzyme to homogeneity was achieved. The apparent molecular mass of the pectinase was 47 kDa, as found by sodium dodecyl sulphate–polyacrylamide gel electrophoresis. The optimum activity of the enzyme was recorded at pH 6.0 and 50 °C. The enzyme retained 80.3% and 79.1% residual activity, respectively at pH 6.0 and 50 °C for 90 min. The pectinase was best functional in the presence of toluene and retained its activity for 30 min. Cu2+ and Co2+ acted as enzyme activators, while Ca2+, β-mercaptoethanol, dimethyl sulfoxide and ethylenediaminetetraacetic acid proved to be the inhibitors. The Km and Vmax values of the pectinase with pectin as substrate were 0.002 mM and 27.39 U/mL, respectively thus indicating the high enzyme affinity towards the substrate. After 30-min treatment of the grape skin with the partially purified enzyme, microscopic observation revealed that a short time of the enzymatic treatment resulted in substantial loss of pigment and shrinkage of the grape skin cells thereby highlighting the high efficiency of the pectinase. The current study implies that the A. terreus FP6 pectinase may be applied as a bio-agent in the food and beverage industries and has the potential to replace harmful solvents by promoting a greener approach to extract plant pigments.
- Subjects
PECTINS; PECTIC enzymes; ORGANIC acids; ASPERGILLUS terreus; MOLECULAR weights; GEL permeation chromatography; DIMETHYL sulfoxide
- Publication
3 Biotech, 2021, Vol 11, Issue 11, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-021-03033-x