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- Title
Histological Examination of Callogenesis in Bisected Protocorm Culture of Pigeon Orchid (Dendrobium crumenatum Swartz).
- Authors
KAEWUBON, Paveena; MEESAWAT, Upatham
- Abstract
Callogenesis is a prerequisite step for the initiation of callus-mediated plant regeneration. Three-month-old protocorms of Dendrobium crumenatum Swartz (pigeon orchid) were bisected transversely and cultured on modified Vacin and Went (VW) solid medium containing 20 g L-1 sucrose, 2 g L-1 Phytagel added with various concentrations of 1-naphthaleneacetic acid (NAA) (0, 0.1, 0.5 mg L-1 ) and 6-benzyladenine (BA) (0, 1, 2 mg L-1 ). The highest percentage of callus formation was obtained from the explant cultured on a modified VW supplemented with 0.5 mg L-1 NAA and 1 mg L-1 BA. The callus originated from the subepidermal layer of the bisected protocorm and subsequently formed nodular structure after culture for 8 weeks. The callus mass contained small isodiametric cells with prominent nuclei and nucleoli within dense cytoplasm.
- Subjects
UNSCENTED dendrobium; REGENERATION (Biology); PLANTS; CALLUS (Botany); HISTOLOGY; CYTOPLASM
- Publication
Walailak Journal of Science & Technology, 2016, Vol 13, Issue 9, p745
- ISSN
1686-3933
- Publication type
Article